Horng-Huei Liou

 

 

Horng-Huei Liou

Liou, Horng-Huei

MD., Ph.D., National Taiwan University

Address: Room 44, 11F, College of Medicine, NTU.

TEL: (02)23123456 -88325

FAX: (02)23915297

email to Dr. Liou

 

專長 成果 研究方向   重點設備
 

 

Specialties

1] Molecular mechanism for regulation of inwardly rectifying potassium channels

2] Pathophysiology of epilepsy

3] Pathogenesis of Parkinson’s disease

 


 

Research highlights

We are interested in three subjects. The first is molecular mechanism for regulation of inwardly rectifying potassium (Kir) channels. Potassium channels are widely present and regulate many important cellular processes, and are closely related to epilepsy. We are focus on the Kir channels. Recent evidence implicates phosphatidylinostinol 4,5-bisphosphate (PIP2) as a regulator of Kir channels. We use the Xenopus oocyte expression system to examine the possible roles of PIP2 in cloned and mutant Kir channels by means of giant patch clamp technique. We found PKA activates Kir channels by enhancing PIP2 –channel interaction. We will combine molecular biological, biochemical and electrophysiological approach to investigate the structure and function in Kir channels in the future. Our second topic of interest is epilepsy. Clinically, we have developed a means of surgical intervention for patients with intractable seizure. We precisely localize the focus of epileptogenicity and important functional areas in epileptic patients to prevent surgical complication. Basically, we have developed the animal model for status epilepticus (SE). We investigate the regulation of GABAergic synaptic response on dentate gyrus during SE. We performed the whole-cell patch clamp studies in brain-slice. The evoked IPSCs, miniature IPSCs, EPSCs, and miniature EPSCs were recorded in the hippocampus slice in vitro. Our goal is to explore the pathophysiological alteration of SE. The third one of our interest is the pathogenesis of Parkinson’s disease (PD). We have found that environmental factors, especially exposure to paraquat and other herbicides and pesticides, may play important roles in the development of PD in Taiwan. We have further studied the effect of paraquat on the substantia nigra in rats by intracerebral injection of paraquat. Pharmacological, morphological and behavioral observations indicated that intranigrally injected paraquat may possess marked neurotoxicity and induce degeneration of the rat nigrostriatal dopaminergic system. We plan to investigate the mechanism of the involvement of paraquat in PD by means of biochemical and electrophysiological studies.

 近期代表著作:

1] Protein kinase C mediated pHi-regulation of ROMK1 channels via a hosphatidylinositol- 4,5-bisphosphate-dependent. J Mol Model. 18(7): 2929-2941, 2012.   [Abstract]

2] Lovastatin modulates glycogen synthase kinase-3ß pathway and inhibits mossy fiber sprouting after pilocarpine-induced status epilepticus. Plos One. 7(6): e38789, 2012.   [Abstract]

3] Carisbamate (RWJ-333369) inhibits glutamate transmission in the granule cell of the dentate gyrus. Neuropharmacology. 61(8): 1239-1247, 2011.   [Abstract]

4] Levetiracetam inhibits glutamate transmission through presynaptic P/Q-type calcium channels on the granule cells of the dentate gyrus. Brit J Pharmacol. 158(7): 1753-1762, 2009.    [Abstract]

5] Gabapentin activates ROMK1 channels by a protein kinase A (PKA)-dependent mechanism. Brit J Pharmacol. 154(1): 216-225, 2008.  [Abstract]

 

 


Projects

1] We use the Xenopus oocyte expression system to examine the possible roles of PIP2 in cloned  and mutant Kir channels by means of giant patch clamp technique. We will combine molecular biological, biochemical and electrophysiological approach to investigate the structure and function in Kir channels in the future.

2] We performed the whole-cell patch clamp studies in brain-slice. The evoked IPSCs, miniature IPSCs, EPSCs, and miniature EPSCs were recorded in the hippocampus slice in vitro.

 


Facilities

Axopatch 200 patch clamp

 

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