MD., Ph.D., National Taiwan University
Address: Room 44, 11F, College of Medicine, NTU.
TEL: (02)23123456 -88325
Molecular mechanism for regulation of inwardly rectifying potassium
Pathophysiology of epilepsy
3] Pathogenesis of Parkinson’s disease
are interested in three subjects. The first is molecular mechanism for
regulation of inwardly rectifying potassium (Kir) channels. Potassium
channels are widely present and regulate many important cellular
processes, and are closely related to epilepsy. We are focus on the Kir
channels. Recent evidence implicates phosphatidylinostinol
4,5-bisphosphate (PIP2) as a regulator of Kir
channels. We use the Xenopus oocyte expression system to examine
the possible roles of PIP2 in cloned and mutant Kir
channels by means of giant patch clamp technique. We found PKA activates
Kir channels by enhancing PIP2
–channel interaction. We will combine molecular biological,
biochemical and electrophysiological approach to investigate the
structure and function in Kir channels in the future. Our second topic
of interest is epilepsy. Clinically, we have developed a means of
surgical intervention for patients with intractable seizure. We
precisely localize the focus of epileptogenicity and important
functional areas in epileptic patients to prevent surgical complication.
Basically, we have developed the animal model for status epilepticus
(SE). We investigate the regulation of GABAergic synaptic response on
dentate gyrus during SE. We performed the whole-cell patch clamp studies
in brain-slice. The evoked IPSCs, miniature IPSCs, EPSCs, and miniature
EPSCs were recorded in the hippocampus slice in vitro. Our goal
is to explore the pathophysiological alteration of SE. The third one of
our interest is the pathogenesis of Parkinson’s disease (PD). We have
found that environmental factors, especially exposure to paraquat and
other herbicides and pesticides, may play important roles in the
development of PD in Taiwan. We have further studied the effect of
paraquat on the substantia nigra in rats by intracerebral injection of
paraquat. Pharmacological, morphological and behavioral observations
indicated that intranigrally injected paraquat may possess marked
neurotoxicity and induce degeneration of the rat nigrostriatal
dopaminergic system. We plan to investigate the mechanism of the
involvement of paraquat in PD by means of biochemical and
Protein kinase C mediated pHi-regulation of ROMK1 channels via a
J Mol Model.
18(7): 2929-2941, 2012.
Lovastatin modulates glycogen synthase kinase-3ß pathway and inhibits
mossy fiber sprouting after pilocarpine-induced status epilepticus.
7(6): e38789, 2012.
3] Carisbamate (RWJ-333369) inhibits glutamate transmission in the granule cell of the dentate gyrus. Neuropharmacology. 61(8): 1239-1247, 2011. [Abstract]
Levetiracetam inhibits glutamate transmission through presynaptic
P/Q-type calcium channels on the granule cells of the dentate gyrus.
Brit J Pharmacol.
158(7): 1753-1762, 2009.
Gabapentin activates ROMK1 channels by a protein kinase A (PKA)-dependent
Brit J Pharmacol.
We use the Xenopus oocyte expression system to examine the
possible roles of PIP2 in
cloned and mutant Kir channels by means of giant patch clamp
technique. We will combine molecular biological, biochemical and
electrophysiological approach to investigate the structure and function
in Kir channels in the future.
2] We performed the whole-cell patch clamp studies in brain-slice. The evoked IPSCs, miniature IPSCs, EPSCs, and miniature EPSCs were recorded in the hippocampus slice in vitro.
Axopatch 200 patch clamp