姚宗珍 副教授

 

 

Chung-Chen Jane Yao,   Associate Professor

 

國立臺灣大學 牙醫學士

美國加州大學舊金山分校 口腔生物學博士

美國加州大學舊金山分校 矯正住院醫師專科訓練

 

BDS, National Taiwan University, 1989

Ph.D., Oral Biology, University of California San Francisco, 1997

Certificate, Postgraduate in Orthodontics at UCSF, 1999

 

聯絡電話: 23123456-67689
傳真號碼: 23831346
E-Mail: janeyao@ntu.edu.tw


主要研究領域

Tissue- and cellular response to forces

A. Distraction Osteogenesis

Severe sagittal jaw discrepancy found in some patients with craniofacial anomalies has to rely on orthognathic surgery to correct this jaw size problem. Recently, distraction osteogenesis has become a choice to treat patients with severe skeletal discrepancy for better stability with larger degree of advancement. However, we know little of mechanisms of soft tissue response following distraction osteogenesis besides bone can grow in the osteotomy sites in head region. In this study, miniature pigs are used as an animal model. Our ultimate goal is to develop ways to augment soft tissues besides bone structures with mechanical stimulation from distraction osteogenesis.

B. The rate of tooth movement determines the duration of orthodontic treatment. Orthodontic force on tooth induces bone resorption on the compression side and bone deposition on the tension side, thus bone remodels and then tooth moves. Therefore, bone resorption is the rate-limiting step of a lengthy orthodontic treatment.

Bone resorption is a complex process. We simulate part of the bone resorption process by cultivating osteocyte-like cells in three-dimension collagen gel under periodical compression to: 1. Optimize the regulation by changing magnitude and frequency of pressure.

2. Screen for genes similarly regulated by compression force.       To understand the mechanism of bone resorption upon compression is important for controlling bone turnover during orthodontic treatment. Speeding up the rate of tooth movement will help to decrease the cost of expensive orthodontic treatment and the incidence of caries and periodontitis among orthodontic patients.

C. The role of beta1 integrin in force response and contraction. How a cell surface molecule can transmit forces and lead to multiple downstream effects? The signaling cascades initiated at cytoplasmic regaion of integrin molecules will be analyzed through site-directed mutagenesis and evaluated by mechanical studies of cultured cells using a flexible polyacrylamide substrate

and fluorescent beads.

 

 

 

 

 

Main Research Interests

組織以及細胞受力之之反應

A. 下顎延伸式新骨再生

在齒顎矯正的領域中常會面臨許多病患除了齒列問題之外,還合併有顎間,甚至顱顏部骨性差異,其中以顱顏畸形病患最為嚴重。目前臨床上應用延伸式新骨再生(distraction osteogenesis)技術,來治療此類骨性差異大的病患,所得到的延長程度及穩定性均較佳。目前下顎骨延長術已被成功廣泛應用於治療下顎發育不良的顱顏畸形病人,成為臨床上治療方式選擇的主流,但對於下顎長期慢性牽引下周圍顏面肌肉組織的變化、生理病理機轉,卻少有人探討。

本實驗主要以迷你豬動物模式探討下顎骨延長時,周邊骨骼肌包含平行與垂直於延長方向者組織變化的情形;最終目標是想知道,顏面部的骨骼肌是否也會因緩慢牽引技術的機械刺激產生組織增生的現象,進而發展出除了硬組織之外,也有促進軟組織再生的方法。

B.牙齒因受力而在齒槽骨內移動,能使牙周組織維持基本的活性,同時能產生最大細胞反應(骨吸收及骨沉積)的力量,其大小和頻率的特性,在牙齒矯正學之中仍是一個未知的課題。目前我們有興趣的是,骨細胞受到多大的力量會發生有效率的骨吸收,以及引發骨吸收的機制為何。想尋找調節這些變化的適當施力的大小和頻率,以及尋找同樣受壓力調控的基因群組。

瞭解骨質代謝機制之一,甚至於加以調控受力後骨質吸收的機轉,臨床可能將有助於加速矯正牙齒冗長的治療過程,以降低昂貴的治療成本,和減少病患在治療中,因口腔衛生不易維護導致蛀牙或牙周病的罹患率。

C. beta1 integrin在收縮及力量傳導的角色。細胞表面的蛋白質分子如何傳導利量的刺激而導致多方面下游的現象?由integrin細胞質之尾端隻訊息傳導鏈將由特定位置之突變而加以特殊的機械力分析。



五年內研究著作

A.           期刊論文

Yao, C.-C., Ziober, B.L., Squillace, R.M., & Kramer, R.H. (1996)  7 integrin mediates cell adhesion and migration on specific laminin isoforms. Journal of

Biological Chemistry 271(41)25598-25603

Yao, C.-C., Ziober, B.L., Sutherland, A.E., & Kramer, R.H. (1996) Laminins promote the locomotion of skeletal myoblasts via the alpha 7 integrin receptor. Journal of Cell

Science 109:3139-3150

Yao, C.-C., Ziober, B.L., Breuss, J., Pytela, R., & Kramer, R.H. (1997) Functional expression of the alpha 7 integrin receptor in differentiated smooth muscle cells.

Journal of Cell Science 110:1477-1487

Ioannis, S.V., Yao, C.-C., Chen, YQ, Ziober, BL, Tsiftsoglou, A.S., & Kramer, R.H. (2001) Transfection of MCF-7 carcinoma cells with human integrin alpha7 cDNA

promotes adhesion to laminin. Archives of Biochemistry and Biophysics 385(1)108-116

Kawano, K., Kantak, S. S., Murai, M., Yao, C.-C., & Kramer, R. H. (2001) Integrin alpha3beta1 engagement disrupts intercellular adhesion. Experimental Cell Research

262, 180-196.

Blanco-Bose, W.E., Yao, C.-C, Kramer, R.H., & Blau H.M. (2001) Purification of mouse primary myoblasts based on alpha7 integrin expression. Experimental Cell

Research 265, 212-220

Klaffky, E, Williams, R., Yao, C.-C., Ziober, B., Kramer, R., Sutherland, A. (2001) Trophoblast-specific Expression and Function of the Integrin alpha7 subunit in the

Peri-implantation Mouse Embryo. Development Biology 239:161-175

Wang, W.-J., Kuo, J.-C., Yao, C.-C. & Chen, R.-H. (2002) DAP-kinase induces apoptosis by suppressing integrin activity and disrupting matrix survival signals.

Journal of Cell Biology 159:1, 169-179

Tseng, H., Yao, C.-C., Chen, M.-H., Huang, C.-Y., Chen, S.-R., Hsieh, M.-H., Hsu, C.-Y., Chen, W.-M., Chang, H.-H. (2002) The effects of distraction osteogenesis on

mandibular growth in growing mini-pigs: a preliminary report on morphological changes. J. Taiwan Assoc. Orthod. 14:3, 5-13

Chen, Y.-J., Chang, H.-F., Tsai, H.-C., Yao, C.-C. (2002) Orthodontic correction of maxillary incisors crowding using the Invisalign system-case report. Chinese Dent J.

21(4):295-304.

Chang ZC, Yao, C.-C., Chang HF, Chen YJ. Cinical application of customized removable and clear orthodontic aligners -case report. Chinese Dental Journal 2003;

22(4)363-370

Wong, W.-P., Wang, C.-I., Wang, D.-M., Hung, M.-C., Yao, C.-C. (2004) Extrusion remodeling in the enchancement of soft and hard tissue profiles prior to implant

placement-A case report. J. Taiwan Assoc. Orthod. 16(3);59-63

Chen-Y.-H., Chen, Y.-J., Cheng, M.-C., Wang, K-.Y., Lin, L.-D., Yao, C.-C. Removal-torque in miniscrews used for orthodontic anchorage-A preliminary

study. J. Taiwan Assoc. Orthod. 16(3);5-11

Yao, C.-C., Wu, C.-B., Wu, H.-Y., Kok, S.-H., Chang, H.-F., Chen, Y.-J. Intrusion of the Overerupted Upper Left First and Second Molars by Mini-implants with

Partial-fixed Orthodontic Appliances: A Case Report. Angle Orthodontist 2004; 74(4):550-557

Chen SK, Chen YJ, Yao, C.-C., Chang HF. Enhanced speed and precision of measurement in a computer-assisted digital cephalometric analysis system. Angle

Orthodontist 2004; 74(4):501-507

Chen YJ, Chen SK, Yao, C.-C., Chang HF. The effects of differences in landmark identification upon the cephalometric measurements in traditional versus

digitized cephalometry. Angle Orthodontist 2004; 74(2):155-161

Chen YJ, Chen KH, Chen SK, Yao, C.-C., Chang HF. A survey on the NTUH orthodontic patients’attitude toward invisible removable aligner. Chinese Dental

Journal 2004 23(3):221-229

Chuen-Mao Yang, Chin-Sung Chien, Chung-Chen Yao, Li-Der Hsiao, Yu-Chen Huang, and Chou-Bing Wu. Mechanical strain induces collagenases-3 (MMP-13)

expression in MC3T3-E1 osteoblastic cells Journal of Biological Chemistry 2004; 279: 22158 – 2216

Chen YJ, Chen SK, Huang HW , Yao CC, Chang HF. Reliability of landmark identification in cephalometric radiography acquired by storage-phosphor imaging

system. (2004) Dento-maxillo-facial Radiology 33:301-306

Hao-Hueng Chang., Hui Tseng , Thomas Mon-Hsian Hsieh, Chi-Ying Huang, Yuan-Hou Chen, Chao-Yu Hsu, Wei-Ming Cheng, Min-Huey Chen, Shu-Ru Chen,

Chung-Chen Jane Yao. The effects of distraction osteogenesis on mandibular growth in growing minipigs: a report on 3-D morphological changes. Plastic and

Reconstructive surgery 2005; 115: 2014-24

Chung-Chen Jane Yao, Jang-Jaer Lee, Hsing-Yu Chen, Zwei-Chieng Jenny Chang, Hsin-Fu Chang, Yi-Jane Chen. Maxillary molar intrusion with fixed appliances and

mini-implant anchorage studied in 3-D Angle Orthodontist 2005;75:754-760

Yi-Jane Chen, Jiiang-Huei Jeng, Chung-Chen Jane Yao, Ming-Huei Chen, Assistant Professor, Lein-Tuan Hou, Wan-Hong Lan. Long-term effect of pulsed Nd:YAG laser

irradiation on cultured human periodontal fibroblasts Lasers in surgery and Medicine 2005; 36(3)225-233

Min-Huey Chen, Jue-Long Wang, Chung-Chen Yao, Yi-Jane Chen and Ching-Chuan Jiang. Relationship of chondrocyte apoptosis to matrix degradation and swelling

potential of osteoarthritic cartilage J of Formosan Medical Association 2005; 104:264-72

Min-Huey Chen, Yi-Jane Chen, Chung-Chen Yao. Degradation of the type II collagen network early in the degeneration of cartilage Chinese Dent J 2004 23(4) 255-265

Yi-Jane Chen, Yuan-Hou Chen, Li-Deh Lin, Chung-Chen Jane Yao Removal torque of miniscrews used for orthodontic anchorage--A preliminary

report The International Journal of Oral & Maxillofacial Implants 2006; 21:283-289

Kuo, J.-C, Wang, W.-J,. Wu, P.-R., Yao, C.-C., Chen, R.-H. The tumor suppressor DAPK inhibits cell motility by blocking integrin-mediated polarity pathway J Cell

Biology 2006;172(4):619-631

α7 integrin expressing human fetal myogenic progenitors have stem cell-like properties and are capable of osteogenic differentiation

Nobuaki Ozeki, Moon Lim, Chung-Chen Yao, Mirek Tolar and Randall H. Kramer Experimental Cell Research 2006; 312(20) 4162-4180

Chen WL. Lin CT. Yao CC. Huang YH. Chou YB. Yin HS. Hu FR. In-vitro effects of dexamethasone on cellular proliferation, apoptosis, and Na+-K+-ATPase activity of

bovine corneal endothelial cells. Ocular Immunology & Inflammation. 14(4):215-23, 2006 Aug.

Yuan-Hou Chen, Hao-Hueng Chang, Yi-Jane Chen, David Lee , Hsien-Hsiung Chiang, Chung-Chen Jane Yao. Root Contact During Insertion of Miniscrews for

Orthodontic Anchorage Increased the Failure Rate—An Animal Study. Clinical Oral Implant Research 2008;19:99-106

Yi-Jane Chen, Hao-Hueng Chang, Chi-Yin Huang, Hsin-Chia Hung, Eddie Lai, Chung-Chen Yao. A retrospective analysis of the failure rate of three different

orthodontic skeletal anchorage systems. Clinical Oral Implant Research 2007;18:768-775

Chang, H. H., Wu, C. B., Chen, Y. J., Weng, C. Y., Wong, W. P., Chen, Y. J., Chang, B. E. and Yao, C. C.* MMP-3 Response to Compressive Forces in Vitro and in Vivo.

J Dental Research 2008;87(7):692-696

Chen, Y.J.,Chang, H.H., Lin, H.Y., Lai, E., Hung, H.C., Yao, C.C.* Stability of miniplates and miniscrews used for orthodontic anchorage with experience from 492

mini-implants. Clinical Oral Implant Research 2008;19:1188-1196

Chung-Chen Jane Yao, Eddie Hsiang-Hua Lai, Jenny Zwei-Chieng Chang, I Chen, Yi-Jane Chen. Comparison of the treatment outcomes between skeletal anchorage and

extraoral anchorage in adult cases with maxillary dentoalveolar protrusion. American Journal of Orthodontics & Dentofacial Orthopedics 2008;134, 615-624

Eddie Hsiang-Hua Lai, Chung-Chen Jane Yao, Jenny Zwei-Chieng Chang, I Chen, Yi-Jane Chen Three-dimensional dental model analysis of treatment outcomes for

adult protrusive maxillary dentition: comparison among headgear and miniscrew- and miniplate-skeletal anchorage. American Journal of Orthodontics & Dentofacial

Orthopedics 2008:134:636-645

Lai, E., Chen, Y., Yao, C. and Chang, J.. A method to transfer planned implant locations to a preorthodontic cast. J Prosthet Dent. 2008;100, 153-6

Lai, E., Liu, J., Chang, J., Tsai, S., Yao, C., Chen, M., Chen, Y. and Lin, C. Radiographic assessment of skeletal maturation stages for orthodontic patients:

hand-wrist bones or cervical vertebrae? J Formos Med Assoc 2008;107, 316-325

 

B.   研討會論文

1998 ASCB Alpha 7 integrin is a differentiation marker for skeletal and smooth muscle. C.-C. Yao, W. E. Blanco-Florez, H. M. Blau, and R. H. Kramer.

 

2000 ASCB Dynamic expression of alpha 7 integrin on primary aortic smooth muscle cells. C.-C. Yao, and R. H. Kramer.

 

2001 Taiwanese Orthodontists Association (TAO), Kao-Shung, Taiwan. The Influence of Distraction Osteogenesis on Growing Minipigs. HuSei Tseng, M.-H. Lan, C.-C. Yao  (Awarded for the best basic research of the year)

 

2002 Taiwanese Orthodontists Association, Taipei, Taiwan

 

Statistical Characterizations of Clinical Cases using Skeletal Anchorage System in the Orthodontic Department of National Taiwan University Hospital. Chi-Ying Huang, Hao-Hueng Chang, Sang-Heng Kok, Hong-Yi Wu, Yi-Jane Chen, Man-Ching Cheng, Yu-Jiuan Shiau, Chung-Chen Yao

 

The Implact from Changes In Arch Width on Stability after Surgical Correction of Class III Malocclusion-Case Report. Yuan-Hou Chen, Chung-Chen Yao, Yi-Jane Chen, Sang-Heng Kok, Kun-Chee Chen.

The Survey of the Attitudes of Orthodontic Patients to Invisalign System. Jenny Zwei-Chieng Chang, Kang-Yee Wang, Kuang-Ho Chen, Chung-Chen Yao, Frank Hsin-Fu Chang, Yi-Jane Chen

 

2003 Taiwanese Orthodontists Association, Tai-Chung, Taiwan

The effects of mandibular Distraction Osteogenesis in Growing Mini-pigs: A Three-Dimensional Study on Morphological Changes of Mandible. Hui Tseng, Chi-Ying Juang, Hao-Hueng Chang, Min-Huey Chen, Shu-Ru Chen, Thomas Mon-Hsian Hsieh, Chao-Yu Hsu, Wei-Ming Cheng, Hui-Jen Hsia, Chung-Chen Jane Yao (Awarded for the poster displace of the year)

 

Differences of Landmark Identification in Digital Images and Conventional Cephalometric Radiographs. Hui-Wen Huang, Chih-Liang Ho, Ming-Hsien Lan, Chung-Chen Jane Yao, Hsin-Fu Frank Chang, Yi-Jane Chen, Su-kuang Chen.

 

Craniofacial Changes in Patients with Maxillary Deficiency Following Face-mask Therapy with or without a Palatal Expander. Chia-Wei Tsai, Hong-Po Chang, Yi-Jane Chen, Chung-Chen Jane Yao, Fon-lan Chan, Yu-Jiuan Shiau, Hsin-Fun Chang

 

The Clinical Application of Removable and Clear Orthodontic Aligners. Chu-Ying Weng, Yuan-Hou Chen, Wen-Pei Wong, Jenny Zwei-Chieng Chang, Chung-Chen Yao, Hsin-Fu Chang, Yi-Jane Chen

 

The Periodontal Changes Following Orthodontic Intrusive Movement by Bony Anchorage. Shih-Jaw Tsai, Kang-Yee Wang, Man-Ching Cheng, Chung-Chen Jane Yao, Hsin-Fu frank Chang, Yi-Jane Chen

 

A 3-Dimenstional Analysis of Maxillary Molar Intrusion with Fixed Orthodontic Appliance and Bony Anchorage. Hsing-Yu Chen, Wen-Chung Chang, I-Ling Hon, Min-Chih Hung, Chung-Chen Jane Yao, Hsin-Fu Frank Chang, Yi-Jane Chen

 

Subcondylar Fracture Reduction Combined with Orthognathic Surgery-Two Case Reports. Wen-Pei Wong, Chi-Ying Huang, Zwei-Chieng Chang, Hao-Hueng Chang, Thomas Mon-Hsian Hsieh, Yunn-Jy Chen, Chung-Chen Jane Yao (Awarded for the best Oral Presentation of the Year)